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TitlePaired helical filament from primary age-related tauopathy brain
AuthorsShi Y, Murzin AG
MethodHelical(Reported Resolution 2.76 Å)PDB:7nrq
EMDB image
Primary CitationCryo-EM structures of tau filaments from Alzheimer's disease with PET ligand APN-1607. Shi Y, Murzin AG, Falcon B, Epstein A, Machin J, Tempest P, Newell KL, Vidal R, Garringer HJ, Sahara N, Higuchi M, Ghetti B, Jang MK, Scheres SHW, Goedert M Acta Neuropathol (2021) PubMed DOI show all entries for this citation
Funding SourceMedical Research Council (MRC, United Kingdom), Japan Agency for Medical Research and Development (AMED), NIH National Institute on Aging (NIH/NIA), Innovative Medicines Initiative, NIH National Institute of Neurological Disorders and Stroke (NIH/NINDS)
StatusDepositedReleasedLast Update
REL2021-03-042021-03-242024-07-10

Name: Sarkosyl-insoluble fractions from primary age-related tauopathy brainSpecimenType: filament MW: 0.459 MDa pH: 7.5

Component Typestissue
Component Names

Sarkosyl-insoluble fractions from primary age-related tauopathy brain

Molecular Typesprotein
Molecule Names
  • Microtubule-associated protein tau
Sources

Homo sapiens ncbi:9606

Specimen Preparationvitrification
MicroscopeFEI TITAN KRIOS (300 kV)
DetectorGATAN K2 SUMMIT (4k x 4k)
Electron Dose58 (e/sq. Å)
Number of Particles425943

Details: The calibrated pixel size was used during post-processing procedures in RELION.

Map File Size144 Mbytes
Voxel Dimensions (Å)0.82 x 0.82 x 0.82
Map Dimensions (voxels) 330 x 330 x 330
Map Data Type IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Density StatisticsMin -0.053Max 0.106Avg 0Std 0.005
Recommended Contour Level0.0212 (4.0 σ)
Mol*3DViewer Map Visual Analysis @ EMDB
Header Metadataemd-12549.xml emd-12549.cif.gz
Primary Mapemd_12549.map.gz (144 Mbytes)
Masksemd_12549_msk_1.map
Imagesemd_12549.png
FSC Curvesemd_12549_fsc.xml
Half Maps
emd_12549_half_map_2.map.gz
emd_12549_half_map_1.map.gz
Fitted PDB7nrq


Current: files.wwpdb.org (USA)

wwPDB Map Validation Reportemd_12549_full_validation.pdf.gz
wwPDB Model Validation Report7nrq_full_validation.pdf.gz
View EMDB map entry EMD-12549: EMDB PDBj 3Dbionotes
View associated model PDB 7nrq: RCSB-PDB PDBe PDBj