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TitleRelease Complex: BAM bound EspP and Compact SurA
AuthorsFenn KL, Ranson NA
MethodSingle Particle(Reported Resolution 4.3 Å)PDB:8q0g
EMDB image
Primary CitationOuter membrane protein assembly mediated by BAM-SurA complexes. Fenn KL, Horne JE, Crossley JA, Bohringer N, Horne RJ, Schaberle TF, Calabrese AN, Radford SE, Ranson NA Nat Commun (2024) PubMed DOI show all entries for this citation
Funding SourceMedical Research Council (MRC, United Kingdom)
StatusDepositedReleasedLast Update
REL2023-07-282024-10-022024-11-20

Name: Release Complex: BAM bound EspP and Compact SurASpecimenType: particle MW: 0.29 MDa pH: 8

Component Typescomplex
Component Names

Release Complex: BAM bound EspP and Compact SurA

Molecular Typesprotein
Molecule Names
  • Chaperone SurA
  • Outer membrane protein assembly factor BamA
  • Outer membrane protein assembly factor BamB
  • Outer membrane protein assembly factor BamC
  • Outer membrane protein assembly factor BamD
  • Outer membrane protein assembly factor BamE
  • Serine protease EspP
Sources

Escherichia coli ncbi:562

Specimen Preparationvitrification
VitrificationFEI VITROBOT MARK IV
MicroscopeTFS KRIOS (300 kV)
Specimen HolderFEI TITAN KRIOS AUTOGRID HOLDER
DetectorFEI FALCON IV (4k x 4k)
Images Collected29229
Electron Dose37.4 (e/sq. Å)
Number of Particles179199
Fitting ProtocolFLEXIBLE FIT
Fitting Source Models3slt 8pz1 7ttc
Map File Size108 Mbytes
Voxel Dimensions (Å)0.74 x 0.74 x 0.74
Map Dimensions (voxels) 300 x 300 x 300
Map Data Type IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Density StatisticsMin -0.009Max 0.019Avg 0Std 0.001
Recommended Contour Level0.00387 (5.3 σ)
Mol*3DViewer Map Visual Analysis @ EMDB
Header Metadataemd-18053.xml emd-18053.cif.gz
Primary Mapemd_18053.map.gz (108 Mbytes)
Imagesemd_18053.png
FSC Curvesemd_18053_fsc.xml
Half Maps
emd_18053_half_map_2.map.gz
emd_18053_half_map_1.map.gz
Fitted PDB8q0g


Current: files.wwpdb.org (USA)

wwPDB Map Validation Reportemd_18053_full_validation.pdf.gz
wwPDB Model Validation Report8q0g_full_validation.pdf.gz
View EMDB map entry EMD-18053: EMDB PDBj 3Dbionotes
View associated model PDB 8q0g: RCSB-PDB PDBe PDBj