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TitleThree-dimensional model of Salmonella's needle complex at subnanometer resolution
AuthorsSchraidt O, Marlovits TC
MethodSingle Particle(Reported Resolution 8.3 Å)PDB:2y9k
EMDB image
Primary CitationThree-dimensional model of Salmonella's needle complex at subnanometer resolution. Schraidt O, Marlovits TC Science (2011) PubMed DOI show all entries for this citation
StatusDepositedReleasedLast Update
REL2011-01-312011-03-112013-12-11

Name: Needle complex from Salmonella typhimuriumSpecimenType: particle pH: 7.5

Component Typessample
Component Names

Needle complex from Salmonella typhimurium

Molecular Typesprotein
Molecule Names
  • Protein invG
Sources

Salmonella enterica subsp.... ncbi:90371

Specimen Preparation Details: 10mM Tris-HCl 0.5M NaCl 0.1% LDAO

Fitting Details: Protocol: Rigid body. Based on the homolog EscC (PDB entry above) a model was built for InvG (Swissprot - template based modeling), which was subsequently used for the fitting experiment (rigid body, Software CHIMERA)

Specimen Preparationvitrification
VitrificationOTHER
MicroscopeFEI POLARA 300 (300 kV)
Specimen HolderGATAN LIQUID NITROGEN
Imposed SymmetryC15
Reconstruction SoftwareIMAGIC
Fitting ProtocolRIGID BODY FIT
Fitting Source Models3gr5

Details: This in an image of a the surface rendered, extracted volume of the lower neck (15 fold) of the needle complex of the type III secretion system from Salmonella tyhpimurium.

Map File Size105 Mbytes
Voxel Dimensions (Å)1.33 x 1.33 x 1.33
Map Dimensions (voxels) 300 x 300 x 300
Map Data Type IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Density StatisticsMin -0.231Max 0.205Avg 0Std 0.01
Recommended Contour Level0.06 (5.8 σ)
Mol*3DViewer Map Visual Analysis @ EMDB
Header Metadataemd-1871.xml
Primary Mapemd_1871.map.gz (105 Mbytes)
Images1871.jpg
Fitted PDB2y9k


Current: files.wwpdb.org (USA)

wwPDB Map Validation Reportemd_1871_full_validation.pdf.gz
wwPDB Model Validation Report2y9k_full_validation.pdf.gz
View EMDB map entry EMD-1871: EMDB PDBj 3Dbionotes
View associated model PDB 2y9k: RCSB-PDB PDBe PDBj