Status | Deposited | Released | Last Update |
---|
REL | 2011-01-31 | 2011-03-11 | 2013-12-11 |
Name: Needle complex from Salmonella typhimuriumSpecimenType: particle pH: 7.5
Component Types | sample |
---|
Component Names | Needle complex from Salmonella typhimurium |
---|
Molecular Types | protein |
---|
Molecule Names | |
---|
Sources | Salmonella enterica subsp.... ncbi:90371 |
---|
Specimen Preparation Details: 10mM Tris-HCl 0.5M NaCl 0.1% LDAO
Fitting Details: Protocol: Rigid body. Based on the homolog EscC (PDB entry above) a model was built for InvG (Swissprot - template based modeling), which was subsequently used for the fitting experiment (rigid body, Software CHIMERA)
Specimen Preparation | vitrification |
---|
Vitrification | OTHER |
---|
Microscope | FEI POLARA 300 (300 kV) |
---|
Specimen Holder | GATAN LIQUID NITROGEN |
---|
Imposed Symmetry | C15 |
---|
Reconstruction Software | IMAGIC |
---|
Fitting Protocol | RIGID BODY FIT |
---|
Fitting Source Models | 3gr5 |
---|
Details: This in an image of a the surface rendered, extracted volume of the lower neck (15 fold) of the needle complex of the type III secretion system from Salmonella tyhpimurium.
Map File Size | 105 Mbytes |
---|
Voxel Dimensions (Å) | 1.33 x 1.33 x 1.33 |
---|
Map Dimensions (voxels) | 300 x 300 x 300 |
---|
Map Data Type | IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) |
---|
Density Statistics | Min -0.231Max 0.205Avg 0Std 0.01 |
---|
Recommended Contour Level | 0.06 (5.8 σ) |
---|
Mol*3DViewer Map Visual Analysis @ EMDBCurrent: files.wwpdb.org (USA)
View EMDB map entry EMD-1871: | |
---|
View associated model PDB 2y9k: | |
---|