Primary Citation | Visualizing structural transitions of ligand-dependent gating of the TRPM2 channel. Yin Y, Wu M, Hsu AL, Borschel WF, Borgnia MJ, Lander GC, Lee SY Nat Commun (2019) PubMed DOI show all entries for this citation |
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Funding Source | NIH National Institute of Neurological Disorders and Stroke (NIH/NINDS) |
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Status | Deposited | Released | Last Update |
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REL | 2019-06-30 | 2019-08-28 | 2024-10-30 |
Name: Transient receptor potential cation channel subfamily M member 2SpecimenType: particle MW: 0.672 MDa pH: 8
Ligand IDs: CA AR6
Support Details: 15 mA
Reconstruction Details: No symmetry (C1) was imposed during reconstructions and classifications. C2 symmetry was imposed during the final 3D auto-refinement in RELION.
Specimen Preparation | vitrification |
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Vitrification | LEICA EM GP |
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Microscope | FEI TITAN KRIOS (300 kV) |
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Specimen Holder | FEI TITAN KRIOS AUTOGRID HOLDER |
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Detector | FEI FALCON III (4k x 4k) |
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Images Collected | 2496 |
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Electron Dose | 40 (e/sq. Å) |
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Number of Particles | 135120 |
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Imposed Symmetry | C2 |
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Reconstruction Software | RELION |
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Fitting Protocol | RIGID BODY FIT |
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Fitting Source Models | 6d73 |
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Details: zebrafish TRPM2 channel in the presence of ADPR and Ca2+
Map File Size | 67 Mbytes |
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Voxel Dimensions (Å) | 1.39 x 1.39 x 1.39 |
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Map Dimensions (voxels) | 256 x 256 x 256 |
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Map Data Type | IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) |
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Density Statistics | Min -0.167Max 0.306Avg 0Std 0.008 |
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Recommended Contour Level | 0.02 (2.6 σ) |
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Mol*3DViewer Map Visual Analysis @ EMDBCurrent: files.wwpdb.org (USA)
View EMDB map entry EMD-20369: | |
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View associated model PDB 6pkx: | |
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