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TitleCryo-EM structure of the zebrafish TRPM2 channel in the presence of ADPR and Ca2+
AuthorsYin Y, Wu M
MethodSingle Particle(Reported Resolution 4.2 Å)PDB:6pkx
EMDB image
Primary CitationVisualizing structural transitions of ligand-dependent gating of the TRPM2 channel. Yin Y, Wu M, Hsu AL, Borschel WF, Borgnia MJ, Lander GC, Lee SY Nat Commun (2019) PubMed DOI show all entries for this citation
Funding SourceNIH National Institute of Neurological Disorders and Stroke (NIH/NINDS)
StatusDepositedReleasedLast Update
REL2019-06-302019-08-282024-10-30

Name: Transient receptor potential cation channel subfamily M member 2SpecimenType: particle MW: 0.672 MDa pH: 8

Ligand IDs: CA AR6

Support Details: 15 mA

Reconstruction Details: No symmetry (C1) was imposed during reconstructions and classifications. C2 symmetry was imposed during the final 3D auto-refinement in RELION.

Specimen Preparationvitrification
VitrificationLEICA EM GP
MicroscopeFEI TITAN KRIOS (300 kV)
Specimen HolderFEI TITAN KRIOS AUTOGRID HOLDER
DetectorFEI FALCON III (4k x 4k)
Images Collected2496
Electron Dose40 (e/sq. Å)
Number of Particles135120
Imposed SymmetryC2
Reconstruction SoftwareRELION
Fitting ProtocolRIGID BODY FIT
Fitting Source Models6d73

Details: zebrafish TRPM2 channel in the presence of ADPR and Ca2+

Map File Size67 Mbytes
Voxel Dimensions (Å)1.39 x 1.39 x 1.39
Map Dimensions (voxels) 256 x 256 x 256
Map Data Type IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Density StatisticsMin -0.167Max 0.306Avg 0Std 0.008
Recommended Contour Level0.02 (2.6 σ)
Mol*3DViewer Map Visual Analysis @ EMDB
Header Metadataemd-20369.xml emd-20369.cif.gz
Primary Mapemd_20369.map.gz (67 Mbytes)
Imagesemd_20369.png
Half Maps
half map 1emd_20369_half_map_1.map.gz
half map 2emd_20369_half_map_2.map.gz
Fitted PDB6pkx


Current: files.wwpdb.org (USA)

wwPDB Map Validation Reportemd_20369_full_validation.pdf.gz
wwPDB Model Validation Report6pkx_full_validation.pdf.gz
View EMDB map entry EMD-20369: EMDB PDBj 3Dbionotes
View associated model PDB 6pkx: RCSB-PDB PDBe PDBj