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TitleKIF14[391-755] - ADP in complex with a microtubule
AuthorsBenoit MPMH, Asenjo AB
MethodHelical(Reported Resolution 3.4 Å)PDB:6wwj
EMDB image
Primary CitationStructural basis of mechano-chemical coupling by the mitotic kinesin KIF14. Benoit MPMH, Asenjo AB, Paydar M, Dhakal S, Kwok BH, Sosa H Nat Commun (2021) PubMed DOI show all entries for this citation
Funding SourceNIH National Institute of General Medical Sciences (NIH/NIGMS), Canadian Cancer Society Research Institute, Simons Foundation, Canadian Institutes of Health Research (CIHR), NIH Office of the Director, Fonds de Recherche du Quebec-Sante (FRSQ), Agouron Institute, NYSTAR, Natural Sciences and Engineering Research Council (NSERC, Canada)
StatusDepositedReleasedLast Update
REL2020-05-092021-05-052024-05-29

Name: Kif14[391-755] - ADP in complex with a microtubuleSpecimenType: filament MW: 0.144 MDa pH: 6.8

Component Typescomplex, organelle/cell component
Component Names

KIF14[391-755] - ADP

Microtubule

Molecular Typesligand, protein
Molecule Names
  • ADENOSINE-5'-DIPHOSPHATE
  • GUANOSINE-5'-DIPHOSPHATE
  • GUANOSINE-5'-TRIPHOSPHATE
  • Kinesin-like protein KIF14
  • MAGNESIUM ION
  • TAXOL
  • Tubulin alpha-1B chain
  • Tubulin beta-2B chain
Sources

Sus scrofa ncbi:9823

Mus musculus ncbi:10090

Ligand IDs: GTP MG GDP TA1 ADP

Reconstruction Details: 2 half datasets containing one distinct half of each filament were refined independently.

Specimen Preparationvitrification
VitrificationFEI VITROBOT MARK IV
MicroscopeFEI TITAN KRIOS (300 kV)
Specimen HolderFEI TITAN KRIOS AUTOGRID HOLDER
DetectorGATAN K2 SUMMIT (4k x 4k)
Electron Dose70.6 (e/sq. Å)
Number of Particles171108
Reconstruction SoftwareRELION
Fitting ProtocolFLEXIBLE FIT

Details: Main map. Locally refined single unit. Not helically averaged. Composite map from the localdeblur map and the 7 A low pass filtered map.

Map File Size288 Mbytes
Voxel Dimensions (Å)0.831 x 0.831 x 0.831
Map Dimensions (voxels) 416 x 416 x 416
Map Data Type IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Density StatisticsMin -0.006Max 0.077Avg 0Std 0.002
Recommended Contour Level0.00973 (6.4 σ)
Mol*3DViewer Map Visual Analysis @ EMDB
Header Metadataemd-21937.xml emd-21937.cif.gz
Primary Mapemd_21937.map.gz (288 Mbytes)
Masksemd_21937_msk_1.map emd_21937_msk_2.map emd_21937_msk_3.map emd_21937_msk_4.map emd_21937_msk_5.map emd_21937_msk_6.map emd_21937_msk_7.map
Imagesemd_21937.png
FSC Curvesemd_21937_fsc.xml
Additional Maps
Helical reconstruction.emd_21937_additional_2.map.gz
7 A low pass filtered localdeblur map - used to make the composite map. Locally refined single unit. Not helically averaged.emd_21937_additional_5.map.gz
Localdeblur map - used to make the composite map. Locally refined single unit. Not helically averaged.emd_21937_additional_4.map.gz
Helical reconstruction half map 2 (gold standard).emd_21937_additional_3.map.gz
Helical reconstruction half map 1 (gold standard).emd_21937_additional_1.map.gz
Half Maps
Half map 2 (gold standard). Locally refined single unit. Not helically averaged.emd_21937_half_map_2.map.gz
Half map 1 (gold standard). Locally refined single unit. Not helically averaged.emd_21937_half_map_1.map.gz
Fitted PDB6wwj


Current: files.wwpdb.org (USA)

wwPDB Map Validation Reportemd_21937_full_validation.pdf.gz
wwPDB Model Validation Report6wwj_full_validation.pdf.gz
View EMDB map entry EMD-21937: EMDB PDBj 3Dbionotes
View associated model PDB 6wwj: RCSB-PDB PDBe PDBj