Primary Citation | Structural insight into selectivity of amylin and calcitonin receptor agonists. Cao J, Belousoff MJ, Gerrard E, Danev R, Fletcher MM, Dal Maso E, Schreuder H, Lorenz K, Evers A, Tiwari G, Besenius M, Li Z, Johnson RM, Wootten D, Sexton PM Nat Chem Biol (2024) PubMed DOI show all entries for this citation |
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Funding Source | Australian Research Council (ARC), Japan Science and Technology, Takeda Science Foundation, National Health and Medical Research Council (NHMRC, Australia) |
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Status | Deposited | Released | Last Update |
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REL | 2022-11-03 | 2023-08-02 | 2024-10-30 |
Name: calcitonin receptor in complex with Gs protein and San45 peptideSpecimenType: particle MW: 0.177 MDa pH: 7.4
Component Types | complex |
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Component Names | calcitonin receptor in complex with Gs protein and San45 peptide |
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Molecular Types | ligand, protein |
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Molecule Names | - (2S)-2-{[(1R)-1-hydroxyhexadecyl]oxy}-3-{[(1R)-1-hydroxyoctadecyl]oxy}propyl 2-(trimethylammonio)ethyl phosphate
- 2-acetamido-2-deoxy-beta-D-glucopyranose
- CHOLESTEROL HEMISUCCINATE
- Calcitonin receptor
- Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
- Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
- Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
- N-hexadecanoyl-L-glutamic acid
- PALMITIC ACID
- PHOSPHATIDYLETHANOLAMINE
- nanobody 35
- pramlintide analogue San45
- water
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Sources | Lama glama ncbi:9844 Homo sapiens ncbi:9606 |
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Ligand IDs: D6M NAG PTY PLM Y01 P42 HOH
Specimen Preparation | vitrification |
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Microscope | TFS KRIOS (300 kV) |
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Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
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Electron Dose | 40.1 (e/sq. Å) |
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Number of Particles | 636000 |
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Fitting Protocol | AB INITIO MODEL |
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Map File Size | 131 Mbytes |
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Voxel Dimensions (Å) | 0.65 x 0.65 x 0.65 |
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Map Dimensions (voxels) | 320 x 320 x 320 |
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Map Data Type | IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) |
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Density Statistics | Min -0.1Max 0.229Avg 0Std 0.006 |
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Recommended Contour Level | 0.03 (4.7 σ) |
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Mol*3DViewer Map Visual Analysis @ EMDBCurrent: files.wwpdb.org (USA)
View EMDB map entry EMD-28758: | |
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View associated model PDB 8f0j: | |
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