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TitleCalcitonin Receptor in complex with Gs and Pramlintide analogue peptide San45
AuthorsCao J, Sexton PM, Wootten DL, Belousoff MJ
MethodSingle Particle(Reported Resolution 2 Å)PDB:8f0j
EMDB image
Primary CitationStructural insight into selectivity of amylin and calcitonin receptor agonists. Cao J, Belousoff MJ, Gerrard E, Danev R, Fletcher MM, Dal Maso E, Schreuder H, Lorenz K, Evers A, Tiwari G, Besenius M, Li Z, Johnson RM, Wootten D, Sexton PM Nat Chem Biol (2024) PubMed DOI show all entries for this citation
Funding SourceAustralian Research Council (ARC), Japan Science and Technology, Takeda Science Foundation, National Health and Medical Research Council (NHMRC, Australia)
StatusDepositedReleasedLast Update
REL2022-11-032023-08-022024-10-30

Name: calcitonin receptor in complex with Gs protein and San45 peptideSpecimenType: particle MW: 0.177 MDa pH: 7.4

Component Typescomplex
Component Names

calcitonin receptor in complex with Gs protein and San45 peptide

Molecular Typesligand, protein
Molecule Names
  • (2S)-2-{[(1R)-1-hydroxyhexadecyl]oxy}-3-{[(1R)-1-hydroxyoctadecyl]oxy}propyl 2-(trimethylammonio)ethyl phosphate
  • 2-acetamido-2-deoxy-beta-D-glucopyranose
  • CHOLESTEROL HEMISUCCINATE
  • Calcitonin receptor
  • Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
  • Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
  • Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
  • N-hexadecanoyl-L-glutamic acid
  • PALMITIC ACID
  • PHOSPHATIDYLETHANOLAMINE
  • nanobody 35
  • pramlintide analogue San45
  • water
Sources

Lama glama ncbi:9844

Homo sapiens ncbi:9606

Ligand IDs: D6M NAG PTY PLM Y01 P42 HOH

Specimen Preparationvitrification
MicroscopeTFS KRIOS (300 kV)
DetectorGATAN K3 BIOQUANTUM (6k x 4k)
Electron Dose40.1 (e/sq. Å)
Number of Particles636000
Fitting ProtocolAB INITIO MODEL
Map File Size131 Mbytes
Voxel Dimensions (Å)0.65 x 0.65 x 0.65
Map Dimensions (voxels) 320 x 320 x 320
Map Data Type IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Density StatisticsMin -0.1Max 0.229Avg 0Std 0.006
Recommended Contour Level0.03 (4.7 σ)
Mol*3DViewer Map Visual Analysis @ EMDB
Header Metadataemd-28758.xml emd-28758.cif.gz
Primary Mapemd_28758.map.gz (131 Mbytes)
Masksemd_28758_msk_1.map
Imagesemd_28758.png
FSC Curvesemd_28758_fsc.xml
Additional Maps
local refinement for receptor regionemd_28758_additional_1.map.gz
Half Maps
emd_28758_half_map_2.map.gz
emd_28758_half_map_1.map.gz
Fitted PDB8f0j


Current: files.wwpdb.org (USA)

wwPDB Map Validation Reportemd_28758_full_validation.pdf.gz
wwPDB Model Validation Report8f0j_full_validation.pdf.gz
View EMDB map entry EMD-28758: EMDB PDBj 3Dbionotes
View associated model PDB 8f0j: RCSB-PDB PDBe PDBj