Primary Citation | Structural snapshots of V/A-ATPase reveal the rotary catalytic mechanism of rotary ATPases. Kishikawa J, Nakanishi A, Nakano A, Saeki S, Furuta A, Kato T, Mistuoka K, Yokoyama K Nat Commun (2022) PubMed DOI show all entries for this citation |
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Funding Source | Ministry of Education, Culture, Sports, Science and Technology (Japan), Japan Agency for Medical Research and Development (AMED), Japan Society for the Promotion of Science (JSPS) |
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Status | Deposited | Released | Last Update |
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REL | 2021-08-30 | 2022-07-13 | 2024-06-19 |
Name: V1EG of V/A-ATPase from Thermus thermophilus, high ATP, state1-2SpecimenType: particle MW: 0.4 MDa pH: 8
Component Types | complex |
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Component Names | V1EG of V/A-ATPase from Thermus thermophilus, high ATP, state1-2 |
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Molecular Types | ligand, protein |
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Molecule Names | - ADENOSINE-5'-DIPHOSPHATE
- ADENOSINE-5'-TRIPHOSPHATE
- MAGNESIUM ION
- PHOSPHATE ION
- V-type ATP synthase alpha chain
- V-type ATP synthase beta chain
- V-type ATP synthase subunit D
- V-type ATP synthase subunit E
- V-type ATP synthase subunit F
- V-type ATP synthase, subunit G
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Sources | Thermus thermophilus HB8 ncbi:300852 |
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Ligand IDs: ADP MG PO4 ATP
Fitting Details: The atomic model built in this study was used as an initial model.
Specimen Preparation | vitrification |
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Vitrification | FEI VITROBOT MARK IV |
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Microscope | FEI TITAN KRIOS (300 kV) |
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Specimen Holder | FEI TITAN KRIOS AUTOGRID HOLDER |
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Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
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Electron Dose | 50 (e/sq. Å) |
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Number of Particles | 28801 |
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Imposed Symmetry | C1 |
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Reconstruction Software | RELION |
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Fitting Protocol | OTHER |
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Details: V1EG of V/A-ATPase from Thermus thermophilus, state1-2
Map File Size | 108 Mbytes |
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Voxel Dimensions (Å) | 0.88 x 0.88 x 0.88 |
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Map Dimensions (voxels) | 300 x 300 x 300 |
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Map Data Type | IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) |
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Density Statistics | Min -0.062Max 0.125Avg 0Std 0.006 |
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Recommended Contour Level | 0.022 (3.6 σ) |
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Mol*3DViewer Map Visual Analysis @ EMDBCurrent: files.wwpdb.org (USA)
View EMDB map entry EMD-31850: | |
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View associated model PDB 7vam: | |
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