Primary Citation | Structural basis for a conserved neutralization epitope on the receptor-binding domain of SARS-CoV-2. Huang KA, Chen X, Mohapatra A, Nguyen HTV, Schimanski L, Tan TK, Rijal P, Vester SK, Hills RA, Howarth M, Keeffe JR, Cohen AA, Kakutani LM, Wu YM, Shahed-Al-Mahmud M, Chou YC, Bjorkman PJ, Townsend AR, Ma C Nat Commun (2023) PubMed DOI show all entries for this citation |
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Funding Source | Ministry of Science and Technology (MoST, Taiwan) |
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Status | Deposited | Released | Last Update |
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REL | 2022-11-17 | 2023-02-01 | 2024-11-06 |
Name: Complex of Delta Spike and IY-2A with two Fabs bound to one Spike in 3-RBD-up conformationSpecimenType: particle MW: 0.55 MDa pH: 7.5
Component Types | complex |
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Component Names | IY-2A SARS-CoV-2 Delta Spike protein |
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Molecular Types | protein |
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Molecule Names | - IY-2A Fab heavy chain
- IY-2A Fab light chain
- Spike glycoprotein
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Sources | Homo sapiens ncbi:9606 Severe acute respiratory syndrome... ncbi:2697049 |
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Specimen Preparation Details: 20mM Tris pH 7.5
150mM NaCl
Specimen Preparation | vitrification |
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Vitrification | FEI VITROBOT MARK IV |
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Microscope | FEI TITAN KRIOS (300 kV) |
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Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
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Images Collected | 4685 |
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Electron Dose | 1.1 (e/sq. Å) |
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Number of Particles | 67276 |
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Reconstruction Software | RELION |
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Fitting Protocol | FLEXIBLE FIT |
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Map File Size | 136 Mbytes |
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Voxel Dimensions (Å) | 1.06 x 1.06 x 1.06 |
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Map Dimensions (voxels) | 324 x 324 x 324 |
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Map Data Type | IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) |
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Density Statistics | Min -0.026Max 0.044Avg 0Std 0.001 |
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Recommended Contour Level | 0.0037 (2.6 σ) |
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Mol*3DViewer Map Visual Analysis @ EMDBCurrent: files.wwpdb.org (USA)
View EMDB map entry EMD-34808: | |
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View associated model PDB 8hhz: | |
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