Primary Citation | Structural basis for ligand recognition and activation of the prostanoid receptors. Li X, Zhang X, Wen X, Zhang D, Qu C, Miao X, Zhang W, Zhang R, Liu G, Xiao P, Sun JP, Gong W Cell Rep (2024) PubMed DOI show all entries for this citation |
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Funding Source | Ministry of Science and Technology (MoST, China), National Natural Science Foundation of China (NSFC) |
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Status | Deposited | Released | Last Update |
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REL | 2023-12-21 | 2024-02-28 | 2024-11-13 |
Name: U46619-TP-miniGq-scFv16 complexSpecimenType: particle MW: 0.166 MDa pH: 7.5
Component Types | complex |
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Component Names | U46619-TP-miniGq-scFv16 complex |
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Molecular Types | ligand, protein |
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Molecule Names | - (5Z)-7-{(1R,4S,5S,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2-oxabicyclo[2.2.1]hept-5-yl}hept-5-enoic acid
- Antibody fragment scFv16
- Engineered miniGq
- Fusion tag,Thromboxane A2 receptor,LgBiT
- Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
- Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
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Sources | Homo sapiens ncbi:9606 synthetic construct ncbi:32630 |
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Ligand IDs: PUC
Specimen Preparation | vitrification |
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Microscope | FEI TITAN KRIOS (300 kV) |
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Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
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Electron Dose | 55 (e/sq. Å) |
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Number of Particles | 76401 |
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Map File Size | 28 Mbytes |
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Voxel Dimensions (Å) | 1.07 x 1.07 x 1.07 |
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Map Dimensions (voxels) | 192 x 192 x 192 |
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Map Data Type | IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) |
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Density Statistics | Min -0.307Max 0.443Avg 0Std 0.011 |
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Recommended Contour Level | 0.04 (3.5 σ) |
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Mol*3DViewer Map Visual Analysis @ EMDBCurrent: files.wwpdb.org (USA)
View EMDB map entry EMD-38403: | |
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View associated model PDB 8xjo: | |
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