EMDataResource.org
EMD-
links/downloads:
TitleU46619 bound Thromboxane A2 receptor-Gq Protein Complex
AuthorsZhang X, Li X, Liu G, Gong W
MethodSingle Particle(Reported Resolution 3.11 Å)PDB:8xjo
EMDB image
Primary CitationStructural basis for ligand recognition and activation of the prostanoid receptors. Li X, Zhang X, Wen X, Zhang D, Qu C, Miao X, Zhang W, Zhang R, Liu G, Xiao P, Sun JP, Gong W Cell Rep (2024) PubMed DOI show all entries for this citation
Funding SourceMinistry of Science and Technology (MoST, China), National Natural Science Foundation of China (NSFC)
StatusDepositedReleasedLast Update
REL2023-12-212024-02-282024-11-13

Name: U46619-TP-miniGq-scFv16 complexSpecimenType: particle MW: 0.166 MDa pH: 7.5

Component Typescomplex
Component Names

U46619-TP-miniGq-scFv16 complex

Molecular Typesligand, protein
Molecule Names
  • (5Z)-7-{(1R,4S,5S,6R)-6-[(1E,3S)-3-hydroxyoct-1-en-1-yl]-2-oxabicyclo[2.2.1]hept-5-yl}hept-5-enoic acid
  • Antibody fragment scFv16
  • Engineered miniGq
  • Fusion tag,Thromboxane A2 receptor,LgBiT
  • Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
  • Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
Sources

Homo sapiens ncbi:9606

synthetic construct ncbi:32630

Ligand IDs: PUC

Specimen Preparationvitrification
MicroscopeFEI TITAN KRIOS (300 kV)
DetectorGATAN K3 BIOQUANTUM (6k x 4k)
Electron Dose55 (e/sq. Å)
Number of Particles76401
Map File Size28 Mbytes
Voxel Dimensions (Å)1.07 x 1.07 x 1.07
Map Dimensions (voxels) 192 x 192 x 192
Map Data Type IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Density StatisticsMin -0.307Max 0.443Avg 0Std 0.011
Recommended Contour Level0.04 (3.5 σ)
Mol*3DViewer Map Visual Analysis @ EMDB
Header Metadataemd-38403.xml emd-38403.cif.gz
Primary Mapemd_38403.map.gz (28 Mbytes)
Imagesemd_38403.png
Half Maps
emd_38403_half_map_2.map.gz
emd_38403_half_map_1.map.gz
Fitted PDB8xjo


Current: files.wwpdb.org (USA)

wwPDB Map Validation Reportemd_38403_full_validation.pdf.gz
wwPDB Model Validation Report8xjo_full_validation.pdf.gz
View EMDB map entry EMD-38403: EMDB PDBj 3Dbionotes
View associated model PDB 8xjo: RCSB-PDB PDBe PDBj