Primary Citation | Molecular architecture of coronavirus double-membrane vesicle pore complex. Huang Y, Wang T, Zhong L, Zhang W, Zhang Y, Yu X, Yuan S, Ni T Nature (2024) PubMed DOI show all entries for this citation |
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Funding Source | The University Grants Committee, Research Grants Council (RGC) |
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Status | Deposited | Released | Last Update |
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REL | 2024-02-10 | 2024-06-19 | 2024-11-06 |
Name: SARS-CoV-2 nsp3-4 pore complexSpecimenType: particle MW: 0.547 MDa pH: 8
Component Types | complex |
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Component Names | SARS-CoV-2 nsp3-4 pore complex |
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Molecular Types | protein |
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Molecule Names | - Non-structural protein 4
- Papain-like protease nsp3
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Sources | Severe acute respiratory syndrome... ncbi:2697049 |
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Specimen Preparation Details: 150mM NaCl, 10mM Tris-HCl, 1mM EDTA
Imaging Details: The tilt-series were acquired using Thermofisher Krios equipped with a Falcon 4i camera and Selectris energy filter. A dose-symmetric scheme (group of 2) was used, with a tilt range of -51 degree to 51 degree (or -60 to 60) at 3 degree increments and an exposure dose of 3 e/A2 per image. The total dose was 105 or 123 e/A2.
Specimen Preparation | vitrification |
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Vitrification | FEI VITROBOT MARK IV |
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Microscope | TFS KRIOS (300 kV) |
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Detector | TFS FALCON 4i (4k x 4k) |
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Electron Dose | 3 (e/sq. Å) |
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Imposed Symmetry | C6 |
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Reconstruction Software | RELION |
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Fitting Protocol | FLEXIBLE FIT |
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Fitting Software | UCSF Chimera |
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Fitting Source Models | 6wrh 8f2e |
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Details: local resolution map, processed by relion_postprocess
Map File Size | 96 Mbytes |
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Voxel Dimensions (Å) | 1.571 x 1.571 x 1.571 |
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Map Dimensions (voxels) | 288 x 288 x 288 |
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Map Data Type | IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) |
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Density Statistics | Min -2.361Max 2.945Avg 0Std 0.088 |
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Recommended Contour Level | 0.1 (1.1 σ) |
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Mol*3DViewer Map Visual Analysis @ EMDBCurrent: files.wwpdb.org (USA)
View EMDB map entry EMD-39107: | |
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View associated model PDB 8yax: | |
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