Title | Class 1 IP3-bound human type 3 1 4 5-inositol trisphosphate receptor |
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Authors | Hite RK, Paknejad N |
Method | Single Particle(Reported Resolution 3.33 Å)PDB:6dqn |
Primary Citation | Structural basis for the regulation of inositol trisphosphate receptors by Ca2+and IP3. Paknejad N, Hite RK Nat Struct Mol Biol (2018) PubMed DOI show all entries for this citation |
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Status | Deposited | Released | Last Update |
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REL | 2018-06-11 | 2018-08-01 | 2024-10-23 |
Name: human type 3 inositol 1,4,5-trisphosphate receptorSpecimenType: particle MW: 1.22 MDa pH: 8
Component Types | organelle/cell component |
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Component Names | human type 3 inositol 1,4,5-trisphosphate receptor |
Molecular Types | ligand, protein |
Molecule Names |
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Sources | Homo sapiens ncbi:9606 |
Specimen Preparation Details: 150mM Nacl 50mM Tris-HCl, pH 8.0 2mM DTT 0.06% Digitonin 5mM EGTA 50 micromolar IP3
Vitrification Details: Blot for 2 seconds prior to freezing.
Selection Details: Movie frames were aligned with MotionCor2
Specimen Preparation | vitrification |
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Vitrification | FEI VITROBOT MARK IV |
Microscope | FEI TITAN KRIOS (300 kV) |
Specimen Holder | FEI TITAN KRIOS AUTOGRID HOLDER |
Detector | GATAN K2 SUMMIT (4k x 4k) |
Images Collected | 1801 |
Electron Dose | 61 (e/sq. Å) |
Number of Particles | 38777 |
Imposed Symmetry | C4 |
Reconstruction Software | FREALIGN |
Fitting Protocol | AB INITIO MODEL |
Details: Class 1 IP3-bound human type 3 1,4,5-inositol trisphosphate receptor
Map File Size | 226 Mbytes |
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Voxel Dimensions (Å) | 1.088 x 1.088 x 1.088 |
Map Dimensions (voxels) | 384 x 384 x 384 |
Map Data Type | IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) |
Density Statistics | Min -27.584Max 40.044Avg -0.009Std 1.073 |
Recommended Contour Level | 5.13 (4.8 σ) |
Header Metadata | emd-7981.xml emd-7981.cif.gz |
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Primary Map | emd_7981.map.gz (226 Mbytes) |
Images | emd_7981.png |
Fitted PDB | 6dqn |
Current: files.wwpdb.org (USA)
wwPDB Map Validation Report | emd_7981_full_validation.pdf.gz |
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wwPDB Model Validation Report | 6dqn_full_validation.pdf.gz |