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TitleCryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E1-ATP state class2)
AuthorsHiraizumi M, Yamashita K, Nishizawa T, Nureki O
MethodSingle Particle(Reported Resolution 3.22 Å)PDB:6k7iEMPIAR-10303
EMDB image
Primary CitationCryo-EM structures capture the transport cycle of the P4-ATPase flippase. Hiraizumi M, Yamashita K, Nishizawa T, Nureki O Science (2019) PubMed DOI show all entries for this citation
StatusDepositedReleasedLast Update
REL2019-06-072019-08-282024-11-20

Name: ATP8A1-CDC50aSpecimenType: particle MW: 0.173 MDa pH: 7.5

Component Typescomplex
Component Names

ATP8A1-CDC50a

Molecular Typesligand, protein
Molecule Names
  • 2-acetamido-2-deoxy-beta-D-glucopyranose
  • CHOLESTEROL HEMISUCCINATE
  • Cell cycle control protein 50A
  • PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER
  • Phospholipid-transporting ATPase
Sources

Homo sapiens ncbi:9606

Ligand IDs: ACP NAG Y01

Specimen Preparationvitrification
MicroscopeFEI TITAN KRIOS (300 kV)
DetectorGATAN K3 BIOQUANTUM (6k x 4k)
Electron Dose64 (e/sq. Å)
Number of Particles139945
Map File Size70 Mbytes
Voxel Dimensions (Å)0.83 x 0.83 x 0.83
Map Dimensions (voxels) 260 x 260 x 260
Map Data Type IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Density StatisticsMin -0.11Max 0.157Avg 0Std 0.004
Recommended Contour Level0.015 (3.7 σ)
Mol*3DViewer Map Visual Analysis @ EMDB
Header Metadataemd-9934.xml emd-9934.cif.gz
Primary Mapemd_9934.map.gz (70 Mbytes)
Masksemd_9934_msk_1.map
Imagesemd_9934.png
FSC Curvesemd_9934_fsc.xml
Half Maps
emd_9934_half_map_2.map.gz
emd_9934_half_map_1.map.gz
Fitted PDB6k7i


Current: files.wwpdb.org (USA)

wwPDB Map Validation Reportemd_9934_full_validation.pdf.gz
wwPDB Model Validation Report6k7i_full_validation.pdf.gz
View EMDB map entry EMD-9934: EMDB PDBj 3Dbionotes
View associated model PDB 6k7i: RCSB-PDB PDBe PDBj